CDYL antibody (C-Term)

Catalog No. ABIN374294

The Goat Polyclonal anti-CDYL antibody has been validated for WB. It is suitable to detect CDYL in samples from Human, Mouse, Rat, Dog, Cow and Pig.

Quick Overview for CDYL antibody (C-Term) (ABIN374294)

Target: CDYL (Chromodomain Protein, Y-Like (CDYL))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CDYL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDYL Antibody

Binding Specificity: C-Term

Sequence: C-SMLKYLQRKI DEF

Specificity: This antibody detects CDYL at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004815.3, NP_001137442.1, NP_001137443.1 Genename: CDYL

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: 1-3 μg/mL. Approx 75 kDa band observed in Human Testes lysates (calculatedMW of 66.5 kDa according to NP_004815.2). The observed molecular weight corresponds toearlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A.2002 Jun 25,99(13): 8707-12, PMID: 12072557).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CDYL

Target: CDYL (Chromodomain Protein, Y-Like (CDYL))

Alternative Name: CDYL

Background: Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone methylation. Multiple proteins are encoded by transcript variants of this gene.Synonyms: CDY-like, CDYL1, Chromodomain Y-like protein

Gene ID: 9425

NCBI Accession: NP_004815

UniProt: Q9Y232